You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb257303 |
---|---|
Category | Proteins |
Description | Human CD9, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 112 - Ile 195 (Accession # P21926-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 11.6 kDa |
Target | CD9 |
UniProt ID | P21926 |
Protein Sequence | NP_001760.1 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ser 112 - Ile 195 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001760.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 11.6 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD9 protein
Human CD9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 10.5 kDa after removal of the signal peptide. The apparent molecular mass of CD9-His is approximately 10-15 kDa due to glycosylation. | |
Mammalian |
98.00% | |
91.55 kDa (predicted). Due to glycosylation, the protein migrates to 100-110 kDa based on Tris-Bis PAGE result. |
Greater than 90.0% as determined by SDS-PAGE. | |
HEK293 Cells |
≥90% as determined by SDS-PAGE | |
This protein contains the human CD9(Ser112-Ile195) was fused with the C-terminal His Tag and expressed in E. coli. |
> 95%, determined by SDS-PAGE | |
This protein contains the second extracellular domain (Ser 112-Ile 195) of human CD9 (NP_0017601) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells. |