HOMEZ Antibody (N-term)

SKU: orb1938715

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB28306
• Target: This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ.
• Molecular Weight: 61240 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Homeobox and leucine zipper protein Homez, Homeodomain leucine zipper-containing factor, HOMEZ, KIAA1443

Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.

HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).

Quick Database Links

Gene Symbol

This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ.

UniProt

Q8IX15

RefSeq (Protein):NP_065885.2