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Item 1 of 3
HOMEZ Antibody (N-term)
Catalog Number: orb1938715
| Catalog Number | orb1938715 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB28306 |
| Tested applications | FC, IF, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 61240 Da |
| Target | This HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ. |
| UniProt ID | Q8IX15 |
| NCBI | NP_065885.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Homeobox and leucine zipper protein Homez, Homeodo Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.
HOMEZ Antibody (N-term) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HOMEZ Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).
