HCMV pp65 Rabbit Polyclonal Antibody

• Catalog Number: orb10511
• Category: Antibodies
• Description: HCMV pp65 Rabbit Polyclonal Antibody
• Target: UL16
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Predicted Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from HCMV pp65 (251-350/561aa)
• RRID: AB_10747532
• MW: 65 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PP65_HCMVM; PP65_HCMVA; Cytomegalovirus pp65; 65 k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Liu, Xiaoyang et al. Human cytomegalovirus evades antibody-mediated immunity through endoplasmic reticulum-associated degradation of the FcRn receptor Nat Commun, 10, 3020 (2019)

PubMed: 31289263

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cytomegalovirus pp65 Polyclonal Antibody, Unconjugated (orb10511) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.