Goat Anti-Rabbit IgG (H&L) Antibody (HRP)

• Catalog Number: orb1236939
• Category: Antibodies
• Description: Goat Anti-Rabbit IgG (H&L) Antibody (HRP)
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rabbit
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (rAlbumin); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purification: Peroxidase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
• Immunogen: Rabbit IgG - whole molecule
• Tested applications: ELISA, IHC, WB
• Application notes: Peroxidase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:20,000 - 1:100,000, Western Blot 1:2,000 - 1:10,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user).
• Antibody Type: Secondary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only