Insulysin/Insulinase Antibody

• Catalog Number: orb19588
• Category: Antibodies
• Description: Goat polyclonal antibody to IDE
• Target: Insulysin / Insulinase
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QQYNFDRDNTE
• RRID: AB_10746788
• MW: 118, 54
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti insulinase antibody, anti insulysin antibody,
• Note: For research use only
• Entrez: 3416
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 cell lysate using IDE antibody

Western blot analysis of HEK293 lysate using IDE antibody

Immunohistochemical staining of Human Lung using IDE antibody

Immunohistochemical staining of Human Breast using IDE antibody