Insulysin/Insulinase, Biotinylated Antibody (Biotin)

• Catalog Number: orb377877
• Category: Antibodies
• Description: Goat polyclonal antibody against IDE conjugated to Biotin.
• Target: Insulysin / Insulinase, Biotinylated
• Species/Host: Goat
• Conjugation: Biotin
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CQQYNFDRDNTE.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Application notes: WB: Approx 120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.2). Recommended concentration: 0.3-1μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IDE, insulin degrading enzyme, INSULYSIN, Abeta-de
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 lysate using IDE antibody (Biotin)