IDE Antibody, KO Validated

• Catalog Number: orb1274488
• Category: Antibodies
• Description: IDE Antibody, KO Validated
• Target: IDE
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
• UniProt ID: P14735
• MW: Observed: 118kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: insulin-degrading enzyme, FLJ35968, INSULYSIN, ins
• Research Area: Cancer Biology, Cell Biology, Neuroscience, Signal
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IDE antibody (orb1274488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibody (orb1274488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of A549 cells using IDE antibody (orb1274488). Blue: DAPI for nuclear staining.