General Malondialdehyde (MDA) ELISA Kit

• Catalog Number: orb781669
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Malondialdehyde(MDA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Malondialdehyde(MDA).
• Reactivity: All
• Concentration: 2000 ng/mL
• Tested applications: ELISA
• Application notes: standard: 2000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MDA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 31.25-2000 ng/mL
• Sensitivity: 9.47 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: MDA
• Research Area: Gastroenterology & Hepatology, Metabolism Research
• Note: For research use only
• Expiration Date: Please enquire.

References

Adam Bownik 1, Barbara Pawlik-Skowrońska 2 Effects of cyanobacterial oligopeptides: anabaenopeptin-B, aeruginosin-98B and their mixture on oxidative stress biomarkers, HSP70 production and tyrosine phosphatase activity in three aquatic invertebrate species: Brachionus calyciflorus, Thamnocephalus platyurus and Chironomus aprilinus Environ Toxicol Pharmacol, (2025)

PubMed: 40619045