Fos B (phospho Ser27) rabbit pAb Antibody

SKU: orb768230

Description

Rabbit polyclonal antibody to Fos B (phospho-Ser27)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FosB around the phosphorylation site of Ser27. AA range:12-61
• Molecular Weight: 35
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FOSB antibody, anti-G0S3 antibody, anti-Protein fosB antibody, anti-G0/G1 switch regulatory protein 3 antibody

Immunofluorescence analysis of HUVEC cells treated with EGF 200nM 5', using FosB (Phospho-Ser27) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of FosB (Phospho-Ser27) Antibody. The lane on the right is blocked with the FosB (Phospho-Ser27) peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).