Donkey anti-Sheep IgG (H&L), HRP conjugated

• Catalog Number: orb699945
• Category: Antibodies
• Description: Donkey polyclonal antibody against Donkey anti-Sheep IgG (H&L) conjugated to HRP
• Clonality: Polyclonal
• Species/Host: Donkey
• Conjugation: HRP
• Reactivity: Sheep
• Form/Appearance: Lyophilized
• Purity: Immunogen affinity purified donkey IgG.
• Purification: Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of ProClin 150 is used as preservative.For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
• Immunogen: Purified sheep IgG, whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)
• Application notes: Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of ProClin 150 is used as preservative.For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
• Storage: Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.