DIG-11-dUTP

SKU: orb2646501

Description

Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine.\nRecommended Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation: 35% Digoxigenin-11-dUTP/ 65% dTTP\nPlease note: The optimal final concentration of Digoxigenin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Digoxigenin-11-dUTP/dTTP ratio is recommended.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3] and in-house data - Primer Extension with Klenow exo- [4] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [5] - Reverse Transcription with MMLV Reverse Transcriptase [6] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [7], Spectroscopic Properties: λmax 290 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Key Properties

• MW: 1066.91 g/mol (free acid)
• Purity: ≥ 95 % (HPLC)
• Formula: C₄₃H₆₅N₄O₂₁P₃ (free acid)
• SMILES: C1[C@@H]2[C@](CC[C@@H]1OCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@@H]3O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@@H](O)C3)c1)([C@@H]1[C@@H](CC2)[C@@]2([C@]([C@@H](C1)O)([C@H](CC2)C1=CC(=O)OC1)C)O)C

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Digoxigenin-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt, alkali-stable

Compound Identifiers

PubChem CID

146018974