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Cystatin A Antibody / CSTA
Catalog Number: orb2636428
| Catalog Number | orb2636428 |
|---|---|
| Category | Antibodies |
| Description | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | Mouse IgG2b, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Immunogen | A recombinant fragment of human CSTA protein (within amino acids 1-98) was used as the immunogen for the recombinant Cystatin A antibody. |
| UniProt ID | P01040 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application notes | Optimal dilution of the recombinant Cystatin A antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | CSTA/9087R |
| Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Hazard Information | This recombinant Cystatin A antibody is available for research use only. |
| Note | For research use only |
IHC staining of FFPE human tonsil tissue with recombinant Cystatin A antibody (clone CSTA/9087R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant Cystatin A antibody (clone CSTA/9087R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cystatin A antibody (clone CSTA/9087R) as confirmation of integrity and purity.
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