Chloramphenicol

SKU: orb1309420

Description

Chloramphenicol is a broad-spectrum antibiotic that inhibits bacterial protein synthesis by targeting the 50S ribosomal subunit and blocking peptidyl transferase activity. It is widely used in molecular biology and microbiological research, both in vitro and in animal infection models, to study protein synthesis and antibiotic mechanisms.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Infectious Disease & Virology, Metabolism Research, Protein Biochemistry, Signal Transduction

Key Properties

• CAS Number: 56-75-7
• MW: 323.13
• Purity: 99.84%
• Formula: C₁₁H₁₂Cl₂N₂O₅
• SMILES: OCC(NC(=O)C(Cl)Cl)C(O)C1=CC=C(C=C1)[N+]([O-])=O
• Target: HIF/HIF Prolyl-Hydroxylase,Antibiotic,Antibacterial,Akt,VEGFR,ribosome,Apoptosis,Autophagy,JNK,MMP
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (15.47 mM);Ethanol:60 mg/mL (185.68 mM);H2O:1 mg/mL (3.09 mM);DMSO:250 mg/mL (773.68 mM)

Bioactivity

• Target IC50:
  protozoan motility:2.95 ± 0.25 mM
• In Vivo:
  METHODS: To investigate the potential to induce aplastic anemia, Chloramphenicol (2000 mg/kg) and Thiamphenicol (850 mg/kg) were administered by gavage to BALB/c mice once daily for 17 days. RESULTS: Administration of Chloramphenicol and Thiamphenicol at hematotoxic dose levels for 17 days in BALB/c mice was associated with the development of aplastic anemia in humans, inducing reversible anemia but not chronic bone marrow aplasia.
• In Vitro:
  METHODS: Human lung cancer cells A549 and H1299 were treated with Chloramphenicol (1-100 µg/mL) for 3-24 h. Cell viability was detected by MTT assay. RESULTS: In the 3 h treatment group, the viability of A549 and H1299 cells at 100 µg/mL was 97.0±3.9% and 98.1±5.0%, respectively, and no cytotoxicity was observed. A significant reduction in cell viability was observed in the 24 h treatment group. METHODS: Mouse T cell lines A1.1 and IE5 and human T cell lines Jurkat and Jcam were stimulated with Chloramphenicol (100 nmol/L), and the DNA content was measured by Flow cytometry. RESULTS: Chloramphenicol clearly blocked activation-induced cell death (AICD) in all four cell lines, even though these cells were activated by different stimuli.

Storage & Handling

• Storage: keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chloramphenicol, Chlornitromycin, Chloromycetin, 50S ribosome, A549, Akt, Aplastic anaemia, Antibiotic, anaerobic infections, Autophagy, Atg6, Apoptosis, Bacterial, Beclin1, H1299, Hypoxia-inducible factors, HIFs, HIFProlylHydroxylase, HIF-PH, HIF, HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF/HIFProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, Matrix metalloproteinases, Levomycetin, mitochondria stress, MMP, Inhibitor, JNK, inhibit, PKB, PI-3K, SENP-1, Protein kinase B, Vascular endothelial growth factor receptor, VEGFR

Compound Identifiers

PubChem CID

298