CD44v4 Antibody

SKU: orb386023

Description

This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titering of the CD44v4 antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: CD44v4/1219
• Immunogen: An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the CD44v4 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human cervical squamos cell carcinoma with CD44v4 antibody (clone CD44v4/1219). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA buffer for 10-20 min.

IHC testing of FFPE human tongue squamos cell carcinoma with CD44v4 antibody (clone CD44v4/1219). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA buffer for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD44v4 Antibody (clone CD44v4/1219). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P16070