CD38 Antibody

SKU: orb1151546

Description

Mouse monoclonal antibody to CD38

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD38 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG2a, kappa
• Clone No.: rCD38/6447
• Immunogen: A portion of amino acids 200-300 of human CD38 protein was used as the immunogen for the CD38 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human Raji cell lysate using CD38 antibody (clone rCD38/6447).

IHC staining of FFPE human tonsil tissue with CD38 antibody (clone rCD38/6447) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate adenocarcinoma tissue with CD38 antibody (clone rCD38/6447) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD38 antibody (rCD38/6447) as confirmation of integrity and purity.

Negative control: IHC staining of FFPE human brain tissue using CD38 antibody (clone rCD38/6447) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P28907