CD19 Antibody

SKU: orb749433

Description

CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]

Images & Validation

• Tested Applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CVID3/429
• Immunogen: Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with CD19 antibody (clone CVID3/429); Red = isotype control, Blue = CD19 antibody.

Flow cytometry testing of lymphocyte-gated human PBM cells with CD19 antibody (clone CVID3/429); Gray = unstained cells, Violet = anti-CD19 antibody stained cells.

SDS-PAGE analysis of purified, BSA-free CD19 antibody (clone CVID3/429) as confirmation of integrity and purity.

Quick Database Links

UniProt

P15391