CD11c Antibody / ITGAX

SKU: orb749613

Description

Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CD11c antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: ITGAX/1243
• Immunogen: A partial recombinant protein corresponding to amino acids 637-827 from human ITGAX/CD11c was used as the immunogen for the CD11c antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human prostate carcinoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE human lung carcinoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

IHC testing of FFPE human follicular lymphoma with CD11c antibody (clone ITGAX/1243). Note staining of cancer cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD11c Antibody (clone ITGAX/1243). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P20702