CD11c Antibody / ITGAX

• Catalog Number: orb749612
• Category: Antibodies
• Description: Recognizes a protein of 145 kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ITGAX/1242
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A partial recombinant protein corresponding to amino acids 637-827 from the human protein was used as the immunogen for the CD11c antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD11c antibody is available for research use only.
• UniProt ID: P20702
• Storage: Store the CD1°C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD11c antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil with CD11c antibody (clone ITGAX/1242). Note specific staining of dendritic cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD11c Antibody (clone ITGAX/1242). Confirmation of Integrity and Purity of the Antibody.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD11c antibody (clone ITGAX/1242). These results demonstrate the foremost specificity of the ITGAX/1242 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.