Catenin-β (phospho Tyr654) rabbit pAb Antibody

SKU: orb767765

Description

Rabbit polyclonal antibody to Catenin-beta (phospho-Tyr654)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Catenin-beta around the phosphorylation site of Tyr654. AA range:620-669
• Molecular Weight: 75
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CTNNB1 antibody, anti-CTNNB antibody, anti-OK/SW-cl.35 antibody, anti-Catenin beta-1 antibody, anti-Beta-catenin antibody

Western Blot analysis of 293 cells using Phospho-Catenin-β (Y654) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Catenin-beta (Phospho-Tyr654) Antibody.

Western blot analysis of lysates from 293 cells, using Catenin-beta (Phospho-Tyr654) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).