Calregulin rabbit pAb

SKU: orb764709

Description

Rabbit polyclonal antibody to Calregulin

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CALR. AA range:21-70
• Molecular Weight: 48
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CALR antibody, anti-CRTC antibody, anti-Calreticulin antibody, anti-CRP55 antibody, anti-Calregulin antibody, anti-Endoplasmic reticulum resident protein 60 antibody, anti-ERp60 antibody, anti-HACBP antibody, anti-grp60 antibody

Immunofluorescence analysis of Hela cell. 1, Calregulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of various cells using Calregulin Polyclonal Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using CALR Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using CALR Antibody. The picture on the right is blocked with the synthesized peptide.