CD20 Antibody

SKU: orb1806379

Description

Anti-CD20 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 146.32 kDa.

Images & Validation

• Tested Applications: ELISA, FA, FACS, In vivo
• Reactivity: Human, Monkey

Key Properties

• Clonality: Monoclonal
• Target: CD20
• Source/Expression System: CHO
• Molecular Weight: 145.2 kDa
• Purity: >95%
• Purification: Protein A
• Endotoxin: <0.001 EU/ug
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20mM Arginine pH 5.0
• Concentration: Batch dependent
• Disclaimer: For research use only

Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CD20 Reference Antibody (rituximab)is more than 98.97% ;determined by SEC-HPLC.

Immobilized human EGFR His at 2 μg/mL can bind Anti-CD20 Reference Antibody (rituximab);EC50=0.01573/0.01258μg/mL.

Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC87=1.334 μg/mL.

Anti-CD20 Reference Antibody (rituximab)-induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.

Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.

Quick Database Links

Gene Symbol

CD20

UniProt

P11836