Aminoallyl-dUTP-XX-AF647

SKU: orb611224

Description

Aminoallyl-dUTP-XX-AF647 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AF647 (structural analog to Alexa Fluor 647®) is a hydrophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-XX-AF647/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AF647/70-50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 648 nm, λem 671 nm, ε 270.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1604.56 g/mol (free acid); Detected MW: 1603.37 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₆₁H₈₈N₇O₂₉P₃S₂
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC[N+]1=C(C(C)(c2cc(ccc12)S(=O)(=O)O)CCCCS(=O)(=O)[O-])/C=C/C=C/C=C\1/N(c2ccc(cc2C1(C)C)S(=O)(=O)O)CCCS(=O)(=O)O

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only