ACSS2 Antibody

SKU: orb1260338

Description

ACSS2 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 367-606 of human ACSS2 (NP_001229322.1).
• Target: ACSS2
• Molecular Weight: Observed: 78kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Acetyl-coenzyme A synthetase, cytoplasmic, Acetate--CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACSS2, ACAS2

Western blot analysis of extracts of various cell lines, using ACSS2 antibody (orb1260338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat lung using ACSS2 Antibody (orb1260338) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using ACSS2 Antibody (orb1260338) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ACSS2 antibody (orb1260338). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ACSS2

UniProt

Q9NR19