ACACA Recombinant Rabbit Monoclonal Antibody

SKU: orb559159

Description

ACACA Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology, Energy Metabolism, Fatty Acid Oxidation, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 2B5
• Immunogen: KLH conjugated synthetic peptide derived from human Acetyl CoA Carboxylase 1(ACC1)
• Target: ACACA
• Molecular Weight: 265 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACACA_HUMAN; Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha; ACC-alpha; ACAC; ACC1; ACCA;

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (orb559159) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Acetyl CoA Carboxylase 1 (ACC1) Monoclonal Antibody, Unconjugated (orb559159) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Brain Lysates, Lane 2: Human K562 cell Lysates, Primary: Anti-Acetyl CoA Carboxylase 1 (ACC1) (orb559159) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 265kDa, Observed band size: 265kDa.

Quick Database Links

Gene Symbol

ACACA

UniProt

Q13085