INCENP antibody

• Catalog Number: orb153970
• Category: Antibodies
• Description: Rabbit polyclonal antibody to INCENP. In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and passenger, or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term passenger proteins encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein of human INCENP(NP_064623.2).
• Dilution range: WB: 1:500-2000
• Conjugation: Unconjugated
• MW: 120-140kDa
• UniProt ID: Q9NQS7
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Alternative names: anti antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cells using INCENP antibody