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Catalog Number | orb1931327 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB20816 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 83678 Da |
Target | This IMMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 700-729 amino acids from the C-terminal region of human IMMT. |
UniProt ID | Q16891 |
NCBI | NP_006830.2, NP_001093639.1, NP_001093640.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | MICOS complex subunit MIC60, Cell proliferation-in Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of hela cells using IMMT Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IMMT Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMMT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of IMMT Antibody (C-term) in Hela cell line lysates (35 ug/lane). IMMT (arrow) was detected using the purified Pab.
Western blot analysis of IMMT (arrow) using rabbit polyclonal IMMT Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IMMT gene (Lane 2).