IL9 Antibody

• Catalog Number: orb1239508
• Category: Antibodies
• Description: IL9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-IL-9 antibody (orb1239508) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9. The immunogen is located within the last 50 amino acids of IL-9.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 16kDObserved: 22 kD (Post-modification: 4 N-linked glycosylations)
• Target: IL9
• UniProt ID: P15248
• NCBI: NP_000581
• Storage: IL-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Spleen Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-9, orb1239508 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-9 recombinant protein per lane. Antibodies: IL-9, orb1239508 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 22kD.

Western Blot Validation in Tissue and Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-9, orb1239508 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of IL-9 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (orb1239508) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IL-9 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (orb1239508) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.