IL7 Antibody (Biotin)

• Catalog Number: orb1671470
• Category: Antibodies
• Description: IL7 Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-7. Anti-Human IL-7 specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: IL7
• UniProt ID: P13232
• NCBI: P13232
• Storage: Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-7, Lymphopoietin 1(LP-1), pre-B cell f
• Note: For research use only
• Application notes: Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human IL-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7. Western Blot To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7.

To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.