Il6ra Antibody

• Catalog Number: orb1671716
• Category: Antibodies
• Description: Il6ra Antibody
• Clonality: Recombinant
• Clone Number: D7715A7
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Lewis rats with OKT4 cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Il6ra
• UniProt ID: P22272
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD126, 15A7, IL-6Ra, IL6R, Interleukin-6 receptor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-6R antibody D7715A7. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - shaded line) or the r version of D7715A7 (orb1671716 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (D7715A7). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of D7715A7 (orb1671716) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671716- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.