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Catalog Number | orb1259381 |
---|---|
Category | Antibodies |
Description | IL6R Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human IL6R (NP_000556.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 52kDa |
Target | IL6R |
UniProt ID | P08887 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | IL6R, interleukin 6 receptor, HGNC:6019, CD126, IL Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using IL6R antibody (orb1259381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Unconjugated | |
95% | |
39.4 kDa | |
Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1). |
Unconjugated | |
95% | |
65.0 kDa | |
Human IL-6 R alpha, Fc Tag (orb570285) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # P08887-1). |
Unconjugated | |
90% | |
63.9 kDa | |
Mouse IL-6 R alpha Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Glu 357 (Accession # P22272-1). |
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