IL4 Antibody

• Catalog Number: orb1264260
• Category: Antibodies
• Description: IL4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Monkey
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: IL4
• UniProt ID: P05112
• NCBI: P05112
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Interleukin-4, IL-4, B-cell stimulatory factor 1,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

(LEFT) Western blot analysis of IL4 Antibody in WiDr cell line lysates (35 ug/lane). IL4 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of IL4 Antibody in mouse cerebellum cell line lysates (35 ug/lane). IL4 (arrow) was detected using the purified Pab.

IL4 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.