IL3RA/CD123 Antibody

• Catalog Number: orb1248078
• Category: Antibodies
• Description: IL3RA/CD123 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: RQQYECLHYKTD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3kDa according to NP_002174.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 43kDa
• Target: IL3RA / CD123
• UniProt ID: P26951
• NCBI: NP_001254642.1, NP_002174.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IL3RA, interleukin 3 receptor, alpha (low affinity
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 43kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3kDa according to NP_002174.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248078 (2 ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248078 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.