IL33 Antibody

• Catalog Number: orb402322
• Category: Antibodies
• Description: IL33 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human IL33 recombinant protein (Position: A95-T270).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18 kDa
• UniProt ID: O95760
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-33; IL-33; Interleukin-1 family member
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of IL33 using anti-IL33 antibody.Lane 1:recombinant human IL33 protein 1ng.

Sandwich ELISA - Recombinant human IL33 protein standard curve.Use in combination with reagents from Human IL33 ELISA Kit EZ-Set (DIY Antibody Pairs).

IHC analysis of IL33 using anti-IL33 antibody.IL33 was detected in paraffin-embedded section of human tonsil.