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Catalog Number | orb1239657 |
---|---|
Category | Antibodies |
Description | IL33 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | IgG |
Immunogen | IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human IL-33.The immunogen is located within amino acids 50 - 100 of IL-33. |
Concentration | 1 mg/mL |
Dilution range | IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | IL33 |
UniProt ID | O95760 |
NCBI | NP_254274 |
Storage | IL-33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | IL-33 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9o Read more... |
Note | For research use only |
Application notes | IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of IL-33 in human lymph node tissue lysate with IL-33 antibody at (A) 1 and (B) 2 µg/mL.
Immunofluorescence of IL-33 in human lymph node tissue with IL-33 antibody at 20 µg/mL.
Immunofluorescence of IL-33 in Human Lymph Node cells with IL-33 antibody at 20 µg/mL.
ELISA, IF, IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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