Il3 Antibody

• Catalog Number: orb1272456
• Category: Antibodies
• Description: Il3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Rat
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3β (rat IL-3 beta).
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3β (150 ng/mL), a concentration of 0.68 - 1.03 μg/mL of this antibody is required.ELISA:To detect Rat IL-3β by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β..Sandwich:To detect Rat IL-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-3βas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β.Western Blot:To detect Rat IL-3β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Il3
• UniProt ID: P97688
• NCBI: P97688
• Storage: IL-3B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Rat IL-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-3-beta (orb1272455) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3-beta.

To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.