IL2RB (Phospho-Y364) antibody

• Catalog Number: orb393331
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL2RB (Phospho-Y364)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y364 of human CD122 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IL2RB
• Entrez: 25746, 3560, 16185
• UniProt ID: P26896, P16297, P14784
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Interleukin-2 receptor subunit beta; IL-2 receptor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD122 (Phospho-Y364) expression in Raji (A), NIH3T3 (B), rat muscle (C) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 70 kD)

Immunofluorescent analysis of CD122 (Phospho-Y364) staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).