IL27 Antibody

• Catalog Number: orb1239663
• Category: Antibodies
• Description: IL27 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-IL-27 antibody (orb1239663) was raised against a peptide corresponding to 14 amino acids near the center of human IL-27. The immunogen is located within amino acids 80 - 130 of IL-27.
• Concentration: 1 mg/mL
• Dilution range: WB: 2-4 μg/mL.Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 28 kDa Observed: 28 kDa
• Target: IL27
• UniProt ID: Q8NEV9
• NCBI: NP_663634
• Storage: IL-27 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Daudi Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1239663, (A: 2 µg/mL, B: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-27 orb1238893 (4 µg/mL), IL-27 orb1239663 (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane. Antibodies: IL-27 orb1239663, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.