IL25 Antibody (Biotin)

• Catalog Number: orb1272431
• Category: Antibodies
• Description: IL25 Antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17E (Human IL-17E).
• Concentration: batch dependent
• Dilution range: ELISA:Sandwich:To detect hIL-17E by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-17E (XP-5202) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17E.Western Blot:To detect hIL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Biotin
• Target: IL25
• UniProt ID: Q9H293
• NCBI: Q9H293
• Storage: IL-17E antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: IL17E, IL17E, UNQ3120/PRO10272, Interleukin-25, In
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect hIL-17E by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-17E (XP-5202) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17E.Western Blot:To detect hIL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human IL-17E by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human IL-17E (orb1272431) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.

To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml.nbsp; When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.