IL23A Antibody

• Catalog Number: orb1239485
• Category: Antibodies
• Description: IL23A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Equine, Guinea pig, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-IL-23 antibody (orb1239485) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-4 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL, IHC: 5 μg/mL.Antibody validated: Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 21kDObserved: 22 kD
• Target: IL23A
• UniProt ID: Q9NPF7
• NCBI: AAH66268
• Storage: IL-23 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Raji Cells. Loading: 15 µg of lysates per lane. Antibodies: IL-23 orb1239485 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: Absence of blocking peptide, Lane B: Presence of blocking peptide.

Western Blot Validation in Human Cell Lines. Loading: 20 µg of lysates per lane. Antibodies: IL-23 orb1239485, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein. Antibodies: IL-23 orb1239485 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of IL-23 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23 with orb1239485 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry Validation of IL-23 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23 antibody (orb1239485) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IL-23 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23 with orb1239485 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of IL-23 in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-23 antibody (orb1239485) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues (Yan et al., 2019).