IL21 Antibody

• Catalog Number: orb1239478
• Category: Antibodies
• Description: IL21 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-IL-21 antibody (orb1239478) was raised against a peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21.
• Concentration: 1 mg/ml
• Dilution range: WB: 1 μg/mL; ICC: 2 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 18kDObserved: 17 kD
• Target: IL21
• UniProt ID: Q9HBE4
• NCBI: NP_068575
• Storage: IL-21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IL-21 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IL-21 Antibody: Za11, IL-21, Interleukin-21, Za11
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-21 orb1239478, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-21 orb1239478 (1 µg/mL), IL-21 (5 µg/mL), beta-actin (5 µg/mL) and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human HL-60 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-21 orb1239478 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. (A) Absence of blocking peptide (B) Presence of blocking peptide.

Immunocytochemistry Validation of IL-21 in K562 Cells. Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (orb1239478) at 2 µg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Induced Expression Validation of IL-21 expresssion in patients with Crohn's Disease (Monteleone et al, 2007). Enhanced IL-21 was observed in involved but not uninvolvedCD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (orb1239478).

Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al., 2013). (b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT). (c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control. Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody.

Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al., 2007). (A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3). (C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3). Both WB show IL-21 expression increased in patients with Hp infection.