IL1RN Antibody

• Catalog Number: orb1263838
• Category: Antibodies
• Description: IL1RN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-129 amino acids from the Central region of human IL1RN.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: IL1RN
• UniProt ID: P18510
• NCBI: P18510
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Interleukin-1 receptor antagonist protein, IL-1RN,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of IL1RN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of IL1RN using rabbit polyclonal IL1RN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2).

IL1RN Antibody IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.