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Catalog Number | orb750673 |
---|---|
Category | Antibodies |
Description | IL1R2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Human, Primate |
Isotype | Antiserum |
Immunogen | This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II. |
Concentration | 85 mg/mL |
Dilution range | ELISA: 1:1,000 - 1:2,000, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. |
Conjugation | Unconjugated |
UniProt ID | P27930 |
NCBI | P27930.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | IL1RB antibody, Interleukin 1 receptor type II pre Read more... |
Note | For research use only |
Application notes | This antiserum against Human IL-1 Receptor Type II (IL-1RII) has been tested by western blot and is suitable for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1RII has been termed p68 or CD121 in some laboratories. IL-1RII has been shown to bind IL-1, IL-1a and IL-1b. |
Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel).
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