IL1R2 antibody

• Catalog Number: orb750672
• Category: Antibodies
• Description: IL1R2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human, Primate
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:2,000, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.
• Conjugation: Unconjugated
• UniProt ID: P27930
• NCBI: P27930.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: IL1RB antibody, Interleukin 1 receptor type II pre
• Note: For research use only
• Application notes: This antiserum against Human IL-1 Receptor Type II (IL-1RII) has been tested by western blot and is suitable for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1RII has been termed p68 or CD121 in some laboratories. IL-1RII has been shown to bind IL-1, IL-1a and IL-1b.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel).