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Catalog Number | orb2292729 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant IL1B. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2b Kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | IL1B (AAH08678, 170 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Protein Sequence | DKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS |
Tested applications | ELISA, IF, IP, WB |
Clone Number | 2A8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | AAH08678 |
Detection limit for recombinant GST tagged IL1B is approximately 10 ng/ml as a capture antibody.
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell. [antibody concentration 10 ug/ml]
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B MaxPab rabbit polyclonal antibody.
Western Blot detection against Immunogen (36.63 KDa).