IL18R1 Antibody / Interleukin 18 receptor alpha

• Catalog Number: orb1823889
• Category: Antibodies
• Description: Interleukin-18 (IL-18) has been identified as a molecule that induces IFN-Gamma production and enhances NK cell cytotoxicity. IL-18 receptor (IL-18R) is a type I membrane protein present in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta and heart, and absent from brain, skeletal muscle, pancreas and kidney. IL-18R is present in Hodgkin s disease cell lines, and does not bind IL-1Alpha or IL-1beta. The association of IL-18 to IL-18R leads to activation of NFkB. At present two subunits of IL-18R have been characterized: IL-18RAlpha and IL-18Rbeta. IL-18RAlpha has been described as the ligand-binding chain and IL-18Rbeta as the signal-transduction chain.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IL-18R alpha antibody.
• UniProt ID: Q13478
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: IL18R1/7593
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the IL-18R alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This IL-18R alpha antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human adrenal gland tissue with IL-18R alpha antibody (clone IL18R1/7593). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free IL-18R alpha antibody (clone IL18R1/7593) as confirmation of integrity and purity.