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Catalog Number | orb1272476 |
---|---|
Category | Antibodies |
Description | Il18bp Antibody (Biotin) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-18 BP (murine Interleukin-18 Binding Protein). |
Concentration | batch dependent |
Dilution range | ELISA:Sandwich:To detect mIL-18BP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-18BP (XP-5173) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-18BP.Western Blot:To detect mIL-18 BP by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-18 BP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Biotin |
Target | Il18bp |
UniProt ID | Q9Z0M9 |
NCBI | Q9Z0M9 |
Storage | IL-18 Binding Protein antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | MC54L, Igifbp, IL-18BP, Interleukin-18-binding pro Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect mIL-18BP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-18BP (XP-5173) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-18BP.Western Blot:To detect mIL-18 BP by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-18 BP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
WB | |
Bovine, Human, Porcine | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Biotin |
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