IL18 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1974644
• Category: Antibodies
• Description: IL18 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 14E15
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human IL18 protein (1-192/192aa)
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: IL18
• UniProt ID: Q14116
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS (pH7.2) with 40% Glycerol, 0.05% rAlbumin and 0.02% Proclin300.
• Alternative names: Interleukin-18; interferon gamma-inducing factor I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (IL18) Monoclonal Antibody, Unconjugated (orb1974644) at 1:250 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Normal HeLa cell lysates, Lane 2: HeLa cell treated with Brefeldin A (5 μ g/ml, 15 min), Lane 3: Jurkat cell lysates (Negative control), Primary: Anti-IL18 (orb1974644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 23 kDa.