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Catalog Number | orb20245 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IL18 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Human |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 22.3; 21.9 |
Target | IL18 |
Entrez | 3606 |
Protein Sequence | NPPDNIKDTKSDI |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | IL18; interleukin 18 (interferon-gamma-inducing fa Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 23kDa band observed in Human Placenta lysates and in lysates of cell line HeLa (calculated MW of 22.3kDa according to NP_001553.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20245 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICC, IF, IP, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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