IL18 Antibody

• Catalog Number: orb180712
• Category: Antibodies
• Description: IL18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat Flow Cytometry(Fixed), 1-3μg/1x106 cells, Mouse Direct ELISA, 0.1-0.5μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22303 MW
• UniProt ID: P97636
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-18;IL-18;Interferon gamma-inducing fac
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RH35 cells using anti-IL18 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of IL18 using anti-IL18 antibody.Lane 1:mouse spleen tissue; 2:mouse thymus tissue; 3:mouse lung tissue; 4:mouse ANA-1 cell.

IHC analysis of IL18 using anti-IL18 antibody. IL18 was detected in paraffin-embedded section of rat spleen tissue.