IL18 Antibody

• Catalog Number: orb180704
• Category: Antibodies
• Description: IL18 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22326 MW
• UniProt ID: Q14116
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Interleukin-18;IL-18;Iboctadekin;Interferon gamma-
• Note: For research use only
• Application notes: WB: The detection limit for IL-18 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of IL18 using anti-IL18 antibody.IL18 was detected in an immunocytochemical section of blood cells.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

WB analysis of IL18 using anti-IL18 antibody.Lane 1:human HeLa cell.

IHC analysis of IL18 using anti-IL18 antibody. IL18 was detected in a paraffin-embedded section of human tonsil tissue.